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Aging and disease  2018, Vol. 9 Issue (3): 391-400    DOI: 10.14336/AD.2017.0726
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Evaluation of Hyperbaric Oxygen Treatment in Acute Traumatic Spinal Cord Injury in Rats Using Diffusion Tensor Imaging
Sun Wenzhi1, Tan Jiewen2, Li Zhuo3,*, Lu Shibao1,*, Li Man4, Kong Chao1, Hai Yong5, Gao Chunjin3, Liu Xuehua3
1Department of Orthopaedics, Beijing Xuanwu Hospital, Capital Medical University, Beijing 100020, China.
2Department of Hyperbaric Oxygen, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510520, China.
3Department of Hyperbaric Oxygen, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.
4Departments of Radiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.
5Department of Orthopaedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.
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This study aimed to evaluate the therapeutic effect of hyperbaric oxygen (HBO) on acute spinal cord injury (SCI) by measuring the in vivo diffusion tensor imaging (DTI) parameters apparent diffusion coefficient (ADC) and fractional anisotropy (FA) and observing diffusion tensor tractography (DTT) of fiber bundle morphology. The rats were randomly divided into sham-operated (SH), SCI, and SCI and hyperbaric oxygen treatment (SCI + HBO) groups (n = 6 in each group). The Basso-Bettie-Bresnahan (BBB) score was used to evaluate motor function recovery, and DTI was performed on days 3, 7, 14, and 21 after surgery. BBB scores and FA values decreased significantly after SCI, while the two values significantly improved in the SCI + HBO group compared with the SCI group on days 7, 14, and 21. ADC increased significantly on days 14 and 21 postoperatively in the SCI group compared with the SH group but did not significantly differ between the SCI and SCI + HBO groups at any time point. BBB scores had the same variation trend with ADC values and FA values in all three groups. In the SH group, DTT showed a well-organized spinal cord, but the spinal cord showed interruptions at sites of injury after SCI. In conclusion, HBO promotes the recovery of neuronal function after SCI. Parameters of DTI, especially FA, can quantitatively evaluate the efficacy of HBO treatment in SCI, while DTT enables the visualization of the fiber tracking of spinal cord tracts.

Key wordsdiffusion tensor imaging    hyperbaric oxygen    spinal cord injury
收稿日期: 2017-03-22      出版日期: 2018-06-05
. [J]. Aging and disease, 2018, 9(3): 391-400.
Sun Wenzhi, Tan Jiewen, Li Zhuo, Lu Shibao, Li Man, Kong Chao, Hai Yong, Gao Chunjin, Liu Xuehua. Evaluation of Hyperbaric Oxygen Treatment in Acute Traumatic Spinal Cord Injury in Rats Using Diffusion Tensor Imaging. Aging and disease, 2018, 9(3): 391-400.
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Figure 1.  
Figure 2.  
Figure 3.  
3 days0.60 ± 0.050.35 ± 0.080.32 ± 0.05
7 days0.70 ± 0.080.41 ± 0.090.54 ± 0.07
14 days0.70 ± 0.090.34 ± 0.060.47 ± 0.08
21 days0.71 ± 0.040.34 ± 0.040.46 ± 0.05
Table 1  
Figure 4.  
Figure 5.  
ADC (10-9 m2/s)SHSCISCI + HBO
3 days1.08 ± 0.080.97 ± 0.250.95 ± 0.11
7 days1.13 ± 0.131.39 ± 0.231.39 ± 0.52
14 days1.28 ± 0.161.58 ± 0.241.63 ± 0.23
21 days1.25 ± 0.141.63 ± 0.151.63 ± 0.22
Table 2  
Figure 6.  
Figure 7.  
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