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Aging and disease  2019, Vol. 10 Issue (3): 578-591    DOI: 10.14336/AD.2018.0604
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Longevity Effect of Liuwei Dihuang in Both Caenorhabditis Elegans and Aged Mice
Weidong Chen1, Jinzeng Wang1, Jiahao Shi1, Xu Yang3, Ping Yang4, Ning Wang1, Sai Yang1, Tianpei Xie5, Hua Yang1, Mengjie Zhang1, Haiyun Wang1,*, Jian Fei1,2,*
1School of Life Science and Technology, Tongji University, Shanghai 200092, China
2Shenqi Institute for Ethnomedicine, Tongji University, Shanghai 200092, China
3School of Medicine, Tongji University, Shanghai 200092, China
4Shanghai Engineering Research Center for Model Organisms, SRMOC/SMOC, Shanghai 201203, China
5Standard Testing Lab (Shanghai) Co., Ltd., Pudong, Shanghai 201203, China
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Abstract

Liuwei Dihuang (LWDH), a famous traditional Chinese medicine, is widely used in the clinical treatment of aging-related diseases in China. However, its pharmacological mechanisms are not clear. In the present study, we evaluated the lifespan extension effect of LWDH in C. elegans and mice and revealed its underlying mechanisms. The results showed that LWDH significantly extended the lifespan of C. elegans in a dose-dependent manner. LWDH also conferred protection to nematodes against oxidative stress and reduced their fat storage. Genetics analysis and microarray data showed that the longevity effect of LWDH was attributed to the regulation of the innate immune response, proteolysis, lipid metabolism, and the oxidation-reduction process and was dependent on daf-16. Among the six herbs in the formula, Radix Rehmanniae Preparata and Fructus Macrocarpii contributed most to the longevity effect of this medicine, while the other four components had a synergistic effect on the longevity effect of the prescription. The lack of any single herb reduced the efficacy of the complete formula. LWDH also extended the lifespan and reduced both the weight and oxidant stress status in aged mice. Taken together, these results suggested that LWDH might function in a multi-target manner to extend the lifespan in both C. elegans and aged mice, and the best effect was achieved with the complete formula.

Key wordstraditional Chinese medicine    lifespan    model organisms    microarray    signal pathway
收稿日期: 2018-02-22      出版日期: 2019-05-28
引用本文:   
. [J]. Aging and disease, 2019, 10(3): 578-591.
Chen Weidong, Wang Jinzeng, Shi Jiahao, Yang Xu, Yang Ping, Wang Ning, Yang Sai, Xie Tianpei, Yang Hua, Zhang Mengjie, Wang Haiyun, Fei Jian. Longevity Effect of Liuwei Dihuang in Both Caenorhabditis Elegans and Aged Mice. Aging and disease, 2019, 10(3): 578-591.
链接本文:  
http://www.aginganddisease.org/CN/10.14336/AD.2018.0604      或      http://www.aginganddisease.org/CN/Y2019/V10/I3/578
GeneForward SequenceReverse Sequence
sea-1ATTGAGAAGAGCCGGAAGCCTGACACCGTCACGAATGAGG
skr-9TCGACTTGATCGTTGCCTGTCTGCGTCCTTGTCGGTGAT
ctl-2TCCCAGATGGGTACCGTCATGGTCCGAAGAGGCAAGTTGA
F42A10.6TACCAACGGAGTACCATGCCCTTCCTTGCTCGGGTCATCA
cpr-1AGACTACCACGGAGCTGGATTTGGAACAGCGTAGGCAGAG
ugt-41TGCGATCTGCCTTGCATAGTTACTATCTTGTGCCGGCTGC
nhr-68GTAGGAATGGACAAGACTCCCGAAGAGGAGATAACTG
dod-3GTGCATATTGTGGAGCTGCGGTACTTGCACGCTGCGAAAA
lipl-4CTCAAAAAGTGTCGATCTTGAGTTGATGAGGTGAATTGGCGACC
vit-5TCTGGACTCCCAACCGCAATTTGAGCACGGAATGGAAGC
sod-3ATCTACTGCTCGCACTGCTTTGTTCACGTAGGTGGCATGA
mtl-1TGTGAGGAGGCCAGTGAGATTAATGAGCCGCAGCAGTT
act-4GCCACCGCTGCCTCCTCATCCCGGCAGACTCCATACCCAAGAAG
Table 1  
TreatmentMean life span
± SEM (days)
Percentage changeNumber of animalsP value
Control20.41 ± 1.010-58-
LWDH-0.5 mg/ml23.74 ± 0.855916.30%540.061
LWDH-1 mg/ml26.97 ± 1.03132.09%58< 0.001 (***)
LWDH-1.5 mg/ml28.98 ± 1.32141.98%59< 0.001 (***)
LWDH-2 mg/ml29.84 ± 1.54946.19%51< 0.001 (***)
LWDH-3 mg/ml26.85 ± 2.49631.53%40< 0.001 (***)
LWDH-5 mg/ml7.368 ± 1.157-63.90%57< 0.001 (***)
LWDH-10 mg/ml3.393 ± 0.2096-83.38%56< 0.001 (***)
50 μM Mianserin29.06 ± 0.910742.35%51< 0.001 (***)
Table 2  
Figure 1.  
Figure 2.  
Figure 3.  
Figure 4.  
Figure 5.  
Figure 6.  
Figure 7.  
Figure 8.  
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