%0 Journal Article %A Shijie Chen %A Yuezhan Li %A Shuang Zhi %A Zhiyu Ding %A Yan Huang %A Weiguo Wang %A Ruping Zheng %A Haiyang Yu %A Jianlong Wang %A Minghua Hu %A Jinglei Miao %A Jinsong Li %T lncRNA Xist Regulates Osteoblast Differentiation by Sponging miR-19a-3p in Aging-induced Osteoporosis %D 2020 %R 10.14336/AD.2019.0724 %J Aging and disease %P 1058-1068 %V 11 %N 5 %X

The switch between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) plays a key role in aging-induced osteoporosis. In this study, miR-19a-3p was obviously downregulated in BMSCs from aged humans and mice. Overexpressed miR-19a-3p evidently reduced aging-induced bone loss in mice and promoted osteogenic differentiation of BMSCs, while silenced miR-19a-3p manifestly increased aging-induced bone loss in mice and repressed osteogenic differentiation of BMSCs. Hoxa5 was significantly downregulated in the BMSCs from aged mice and contribute to miR-19a-3p-induced osteoblast differentiation as a direct target gene of miR-19a-3p. Furthermore, lncRNA Xist was found as a sponge of miR-19a-3p to repress BMSCs osteogenic differentiation. In conclusion, our study reveals the critical role of the lncRNA Xist/miR-19a-3p/Hoxa5 pathway in aging-induced osteogenic differentiation of BMSCs, indicating the potential therapeutic target for osteoporosis.

%U https://www.aginganddisease.org/EN/10.14336/AD.2019.0724