Cellular and Molecular Biomarkers Indicate Premature Aging in Pseudoxanthoma Elasticum Patients

Tiemann Janina, Wagner Thomas, Vanakker Olivier M, Gils Matthias van, Cabrera José-Luis Bueno, Ibold Bettina, Faust Isabel, Knabbe Cornelius, Hendig Doris

Table 2 Primer sequences used for quantitative real-time PCR.

Gene Protein 5´-3´sequence Reference1 Annealing temperature (°C) Efficiency ß-ACTIN beta-Actin ß-Actin CGCGAGAAGATGACCC ATTGCCAATGGTGATGAC NM_001101 59°C 2.0 GAPDH glyceraldehyde-3-phosphate dehydrogenase GAPDH AGGTCGGAGTCAACGGAT TCCTGGAAGATGGTGATG NM_002046 59°C 1.8 β2M beta-2-microglobulin ß2M TGTGCTCGCGCTACTCTCTCTT CGGATGGATGAAACCCAGACA NM_004048 59°C 2.0 GDF11 growth differentiation factor 11 GDF11 AGGCCATTGGCAGAGCATCGAC GTCCCAGCCGAAAGCCTCAAAG NM_005811.3 63°C 2.0 IGFBP3 insulin-like growth factor binding protein 3 IGFBP3 GCGCCAGGAAATGCTAGTGA GGGAATGTGTACACCCCTGG NM_001013398.1 63°C 1.8