lncRNA Xist Regulates Osteoblast Differentiation by Sponging miR-19a-3p in Aging-induced Osteoporosis

Chen Shijie, Li Yuezhan, Zhi Shuang, Ding Zhiyu, Huang Yan, Wang Weiguo, Zheng Ruping, Yu Haiyang, Wang Jianlong, Hu Minghua, Miao Jinglei, Li Jinsong

Table 1 The primers.

Forward 5’-3’ Reverse 5’-3’ β-actin CTGTCCCTGTATGCCTCTG TGATGTCACGCACGATTT Runx2 ACTTCCTGTGCTCCGTGCTG TCGTTGAACCTGGCTACTTGG Hoxa5 CTCATTTTGCGGTCGCTATCC ATCCATGCCATTGTAGCCGTA Osterix ACCAGGTCCAGGCAACAC GCAAAGTCAGATGGGTAAGTAG miR-19a-3p CTGGAGTGTGCAAATCTATGC GTGCAGGGTCCGAGGT LncRNA Xist AATGGAACGGGCTGAGTTTTAG TCATCCGCTTGCGTTCATAG U6 GCGCGTCGTGAAGCGTTC GTGCAGGGTCCGAGGT Antagomir-19a-3p TCAGTTTTGCATAGATTTGCACA antagomir NC CAGUACUUUUGUGUAGUACAA agomir-19a-3p UGUGCAAAUCUAUGCAAAACUGA pGL3-HOXA5 GGGGTACC GCATCTGAGCG CCGCTCGAG GCTGATCACAGTT pGL3-Xist GGGGTACCCGGCTTGCTCCA CCGCTCGAG TTCAAAACAAAGCA pcDNA3.1-HOXA5 GGGGTACC ATGAGCTCTTATT CCGCTCGAG TCAGGGGCGGAA pcDNA3.1-Xist GGGGTACCCGGCTTGCTCCA CCGCTCGAG TTCAAAACAAAGCA siHOXA5 GCACATTAGTCACGACAAT si_control GCATTAGTCACGACCAAAT siXist GCACACATCTCATTCCATT