Chondroprotective Effects of Combination Therapy of Acupotomy and Human Adipose Mesenchymal Stem Cells in Knee Osteoarthritis Rabbits via the GSK3β-Cyclin D1-CDK4/CDK6 Signaling Pathway

An Xingyan, Wang Tong, Zhang Wei, Yu Hongliang, Chunhua Zhao Robert, Guo Yan, Wang Chunjiu, Qin Luxue, Guo Changqing

Table 1 Primers used in this study.

Gene Primer sequences GSK3β Forward: AGGAACACCAAAGGGAGC Reverse: TACTCCAGGAGACGGCTACA cyclin D1 Forward: TCAAGTGTGACCCGGACTG Reverse: GCTTCTTCCTCCACTTCCCC CDK4 Forward: GATGCGCCAGTTTCTAAGCG Reverse: GGCCAGCTTAACTGTCCCAT CDK6 Forward: CGTGGAAGTTCAGACGTGGA Reverse: AAAGCCTGTCTGGGAAGAGC GAPDH Forward: TTCACCACCATGGAGAAGGC Reverse: CTCGTGGTTCACACCCATCA