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Angelica Sinensis Polysaccharide Suppresses Epithelial-Mesenchymal Transition and Pulmonary Fibrosis via a DANCR/AUF-1/FOXO3 Regulatory Axis
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Weibin Qian, Xinrui Cai, Qiuhai Qian, Dongli Wang, Lei Zhang
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| Table 1 Information of the qPCR primer sequences and silencing RNA sequences. |
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| qPCR primer name | Sequence (5’-3’) |
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| DANCR (Forward) | TCGGAGGTGGATTCTGTTAGG | | DANCR (Reverse) | TCGGTGTAGCAAGTCTGGTGA | | FOXO3 (Forward) | CTCCACCCCTGCTGAGATGAT | | FOXO3 (Reverse) | AGTGAGAACGTTGTCCCGCGCTGG | | GAPDH (Forward) | GCACCGTCAAGGCTGAGAAC | | GAPDH (Reverse) | ATGGTGGTGAAGACGCCAGT | | U6 (Forward) | CTCGCTTCGGCAGCACA | | U6 (Reverse) | AACGCTTCACGAATTTGCGT | | U1 (Forward) | GGGAGATACCATGATCACGAAGGT | U1 (Reverse)
Silencing RNA name | CCACAAATTATGCAGTCGAGTTTCCC
Sequence (5’-3’) | si-AUF1
si-FOXO3 si-NC | UCGACUAUCUGCUCCAAG
TGATCAGCCTCAATCTGCA GACCTACAACTACCTATCA |
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