Molecular Aging Markers in Patients with Klinefelter Syndrome

Pohl Eva, Muschal Sina, Kliesch Sabine, Zitzmann Michael, Rohayem Julia, Gromoll Jörg, Laurentino Sandra

Table 1 Primer sequences used for pyrosequencing and relative telomere length determination.

Target Sequence (5’-3’) ASPA forward biotin-GGAGGAATTTATGGGAATGAGTT reverse AAATAATTTTACCTCCAACCCTATTC sequencing ACCCTATTCTCTAAATCTCA ITGA2B forward biotin-TAAGATTTGATTTTGGTTGGGGGTTTT reverse AACCTTACTCCCAAAAAACTCATTTACA sequencing ACAATATACTCAATACTATACCT PDE4C forward GGGTAGAGGTTTGTAGTAGGT reverse biotin-AACTCAAATCCCTCTC sequencing GGTAGTTATAGTATGATTAGAGT Telomere forward CGGTTTGTTTGGGTTTGGGTTTGGGTTTGGGTTTGGGTT reverse GGCTTGCCTTACCCTTACCCTTACCCTTACCCTTACCCT HBG forward TGTGCTGGCCCATCACTTTG reverse ACCAGCCACCACTTTCTGATAGG