Premature CD4+ T Cells Senescence Induced by Chronic Infection in Patients with Acute Coronary Syndrome
Cao Ming, Ruan Lei, Huang Yi, Wang Jinli, Yan Jinhua, Sang Yu, Li Shanshan, Wang Guan, Wu Xiaofen
Table 2 Designs of primers and probes.
PrimerSequence
RAG2 - forward5'-GCAACATGGGAAATGGAACTG-3'
RAG2 - reversed5'-GGTGTCAAATTCATCATCACCATC-3'
RAG2 - probe5'-(FAM)CCCCTGGATCTTCTGTTGATG TTTGACTGTTT
GTGA (Eclipse)-3'
TREC - forward5'-GGGATGTGGCATCACCTTTG-3'
TREC - reversed5'-AAGAAGAAGAAGAAGAAGG
CTCTG-3'
TREC - probe5'-(FAM)TTCCCCACAGGAGCC CCATTTT
GCC (Eclipse)-3'
SELL - forward5'-CTGCTGGACTTACCATTAT-3'
SELL - reversed5'-TATGGCAACTAAATCTGTG-3'
p16Ink4a - forward5'-CGGAGGCCGATCCAGGTCAT-3'
p16Ink4a - reversed5'-AGCACCACCAGCGTGTCCAG-3'
GAPDH - forward5'- AATCCCATCACCATCTTCC-3'
GAPDH - reversed5'- GGACTCCACGACGTACTCA-3'