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Aging and disease    2015, Vol. 6 Issue (1) : 6-16     DOI: 10.14336/AD.2014.0310
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The Human Trochlear and Abducens Nerves at Different Ages - a Morphometric Study
Ramkumar Muthu1, Sharma Saroj1, Jacob Tony G.1, Bhardwaj Daya N.2, Nag Tapas C.1, Roy Tara Sankar1
1Department of Anatomy, All India Institute of Medical Sciences, New Delhi-110608, India
2Department of Forensic Medicine, All India Institute of Medical Sciences, New Delhi-110608, India
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Abstract  

The trochlear and abducens nerves (TN and AN) control the movement of the superior oblique and lateral rectus muscles of the eyeball, respectively. Despite their immense clinical and radiological importance no morphometric data was available from a wide spectrum of age groups for comparison with either pathological or other conditions involving these nerves. In the present study, morphometry of the TN and AN was performed on twenty post-mortem samples ranging from 12-90 years of age. The nerve samples were processed for resin embedding and toluidine blue stained thin (1&x000B5;m) sections were used for estimating the total number of myelinated axons by fractionator and the cross sectional area of the nerve and the axons by point counting methods. We observed that the TN was covered by a well-defined epineurium and had ill-defined fascicles, whereas the AN had multiple fascicles with scanty epineurium. Both nerves contained myelinated and unmyelinated fibers of various sizes intermingled with each other. Out of the four age groups (12-20y, 21-40y, 41-60y and &x0003E;61y) the younger groups revealed isolated bundles of small thinly myelinated axons. The total number of myelinated fibers in the TN and AN at various ages ranged from 1100-3000 and 1600-7000, respectively. There was no significant change in the cross-sectional area of the nerves or the axonal area of the myelinated nerves across the age groups. However, myelin thickness increased significantly in the AN with aging (one way ANOVA). The present study provides baseline morphometric data on the human TN and AN at various ages.

Keywords stereology      morphometry      ocular motor nerves      myelin thickness     
Corresponding Authors: Roy Tara Sankar   
About author:

present address: Kunming Biomed International, Kunming, Yunnan, 650500, China

Issue Date: 01 February 2015
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Ramkumar Muthu
Sharma Saroj
Jacob Tony G.
Bhardwaj Daya N.
Nag Tapas C.
Roy Tara Sankar
Cite this article:   
Ramkumar Muthu,Sharma Saroj,Jacob Tony G., et al. The Human Trochlear and Abducens Nerves at Different Ages - a Morphometric Study[J]. Aging and disease, 2015, 6(1): 6-16.
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http://www.aginganddisease.org/EN/10.14336/AD.2014.0310     OR     http://www.aginganddisease.org/EN/Y2015/V6/I1/6
Figure 1.  Cross-sections of the trochlear nerve in various age groups. Panel of low magnification (left column) and high magnification (right column) photomicrographs of toluidene blue stained semithin resin sections of trochlear nerves showing the Epineurium (Epi), Perineurium (Peri) and a bundle of Small Thinly Myelinated Axons (STMA). The darkly stained profiles seen in the micrographs are the myelin sheaths surrounding the axons of the trochlear nerve. A. and B. are of a 14 year old female; C. and D. are of a 38 year old female; E. and F. are of a 51 year old male and G. and H. are of a 71 year old male. Scale bar: A = C = E = G = 300 &x003BC;m. B = D = F = H = 50 &x003BC;m.
Figure 2.  Cross-sections of the abducens nerve in various age groups. Panel of low magnification (left column) and high magnification (right column) photomicrographs of toluidene blue stained semithin resin sections of trochlear nerves showing the Epineurium (Epi) and Perineurium (Peri). The darkly stained profiles seen in the micrographs are the myelin sheaths surrounding the axons of the abducens nerve. A. and B. are of a 16 year old female; C. and D. are of a 24 year old female; E. and F. are of a 45 year old male and G. and H. are of a 61 year old man. Scale bar: A = 500 &x003BC;m. C = E = G = 300 &x003BC;m. B = D = F = H = 50 &x003BC;m.
Age Groups (Years)No. of samplesMean cross sectional area of nerve (mm2)(SD)Mean estimated myelinated fibers (SD)Mean axonal area of myelinated fibers (&x003BC;m2) (SD)Axonal Diameter (&x003BC;m)Mean myelin thickness (&x003BC;m)(SD)Percentage distribution of fibers (&x00025;)
SmallMediumLarge
A (0-20)50.26 (0.05)2280 (683.37)22.69 (7.24)5.371.84 (0.88)25.6130.4943.9
B (21-40)50.27 (0.08)1920 (294.96)23.27 (7.36)5.442.42 (0.71)#13.6827.3758.95
C (41-60)50.28 (0.08)2400 (758.29)24.13 (3.38)5.542.46 (0.53)#12.1926.8360.98
D (&x0003E;60)50.23 (0.06)2140 (541.29)19.45 (2.64)4.982.48 (0.39)#17.9535.946.15
Table 1:  Morphometric parameters of human trochlear nerve
Age Groups (Years)No. of samplesMean cross sectional area of nerve (mm2) (SD)Mean estimated myelinated fibersMean axonal area of myelinated fibers (&x003BC;m2) (SD)Axonal Diameter (&x003BC;m)Mean myelin thickness (&x003BC;m) (SD)Percentage distribution of fibers(&x00025;)
SmallMediumLarge
A (0-20)50.86 (0.15)5380 (861.39)24.57 (5.91)5.592.78(0.51)8.4531.6959.86
B (21-40)50.95 (0.36)5120 (1145.43)26.93 (5.53)5.852.79 (0.44)5.8433.5860.58
C (41-60)50.67 (0.33)4600 (1662.83)24.22 (4.39)5.552.68 (0.52)*9.2728.8761.86
D(&x0003E;60)50.78 (0.32)4580 (1845.81)23.72 (6.01)5.492.90 (0.51)*8.8330.3960.78
Table 2:  Morphometric parameters of human abducens nerve
Supplementary Figure 1.  Print screen of Optical fractionator and Nucleators in StereoInvestigator Software. This figure illustrates the optical fractionator and the nucleator methods. The unbiased counting frame is used with the fractionator probe. Axonal profiles contained either within the frame or touching the green lines were counted; whereas those that touched the &x02018;forbidden&x02019; red lines were excluded. For the nucleator, a uniform random point within the profile of the axon is used to generate isotropic rays (in yellow) and the distances from the sampled point to the boundaries of the axon profile are marked (as depicted diagramtically in the left inset).
Supplementary Figure 2.  Print Screen showing the morphometric program workflow in StereoInvestigator Software. This figure illustrates the nerve sectional profile contour (N) showing the unbiased counting frame (F) and the sampling grid (G). The contour was traced at a magnification of 100X using a BX61 motorized Olympus microscope. The ideal sampling grid size for this study was determined to be 250&x000B5;m &x000D7; 250&x000B5;m and the unbiased counting frame was 25&x000B5;m &x000D7; 25&x000B5;m. The axon profiles within the unbiased counting frame or touching its green lines were counted, whereas those that touched the &x02018;forbidden&x02019; red lines were excluded.
[1] GlimcherPW2003Eye movementsSquireLRBloomFEMcConnelSKRobertsJLSpitzerNCZigmondMJFundamental Neuroscience2nd EdnNew YorkAcademic Press873892
[2] CalisanellerTOzdemirOAltinorsN2006Posttraumatic acute bilateral abducens nerve palsy in a childChilds Nerv Syst22726728
[3] DwarakanathSGopalSVenkataramanaNK2006Post-traumatic bilateral abducens nerve palsyNeurol India54221222
[4] KurbanyanKLessellS2008Intracranial hypotension and abducens palsy following upper spinal manipulationBr J Ophthalmol92153155
[5] Hanu-CernatLMHallT2009Late onset of abducens palsy after Le Fort I maxillary osteotomyBr J Oral Maxillofac Surg47414416
[6] BrazisPW1993Palsies of the trochlear nerve: diagnosis and localization- recent conceptsMayo Clin Proc68501509
[7] SharmaSRayBBhardwajDDwivediAKRoyTS2009Age changes in the human oculomotor nerve. A stereological studyAnn Anat191260266
[8] JacobsonDMMosterMLEggenbergerERGalettaSLLiuGT1999Isolated trochlear nerve palsy in patients with multiple sclerosisNeurology53877879
[9] BarrDKupersmithMJTurbinRBoseSRothR2000Isolated sixth nerve palsy: an uncommon presenting sign of multiple sclerosisJ Neurol247701704
[10] PetersGB3rdBakriSJKrohelGB2002Cause and Prognosis of nontraumatic sixth nerve palsies in young adultsOphthalmology10919251928
[11] KeaneJR1993Fourth nerve palsy: historical review and study of 215 inpatientsNeurology4324392443
[12] AnwarSNallaSFernandoDJ2008Abducens nerve palsy as a complication of lumbar punctureEur J Intern Med19636637
[13] AdamsMELinnJYousryI2008Pathology of the Ocular Motor Nerves III, IV, and VINeuroimag Clin N Am18261282
[14] JeffersonG1947Isolated oculomotor palsy caused by intracranial aneurysmProc R Soc Med40419432
[15] RuckerCW1966The causes of paralysis of the third, fourth and sixth cranial nervesAm J Ophthalmol6112931298
[16] BurgerLJKalvinNHSmithJL1970Acquired lesions of the fourth cranial nerveBrain93567574
[17] FerreiraTVerbistBvan BuchemMvan OschTWebbA2010Imaging the ocular motor nervesEuro J Radiol74314322
[18] SterioDC1984The unbiased estimation of number and sizes of arbitrary particles using the disectorJ Microsc134127136
[19] LarsenJO1998Stereology of nerve cross sectionsJ Neurosci Meth85107118
[20] HowardCVReedMG1998Random sampling and random geometryUnbiased stereology- three dimensional measurement microscopySpringer-VerlagNew York, NY1933
[21] MayhewTMSharmaAK1984Sampling schemes for estimating nerve fiber size. I. Methods for nerve trunks of mixed fascicularityJ Anat1394558
[22] MayhewTMSharmaAK1984Sampling schemes for estimating nerve fiber size. II. Methods for unifascicular nerve trunksJ Anat1395966
[23] GundersenHJG1977Notes on estimation of numerical density of arbitrary profiles: the edge effectJ Microsc111219223
[24] GundersenHJG1988The nucleatorJ Microsc15321
[25] GundersenHJG1986Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R ThompsonJ Microsc143345
[26] MayhewTM1988An efficient sampling scheme for estimating fiber number from nerve cross sections: The fractionatorsJ Anat157127134
[27] RayBRoyTSWadhwaSRoyKK2005Development of the human fetal cochlear nerve: a morphometric studyHear Res2027486
[28] SchmitzCHofPR2005Design-based stereology in neuroscienceNeuroscience130813831
[29] MillerNR2005Walsh and Hoyts Clinical Neuro-Ophthalmology1Williams &x00026; WilkinsBaltimore, MD9691040
[30] CaramelJPBonnelFRabischongP1983The oculomotor nerves: biometry and endoneural fascicular systematizationAnat Clin5159168
[31] ThomasPK1963The connective tissue of peripheral nerve: an electron microscope studyJ Anat973544
[32] AlltGLawrensonJG2000The blood-nerve barrier: enzymes, transporters and receptors--a comparison with the blood-brain barrierBrain Res Bull52112
[33] SunderlandSHughsESR1946The pupilloconstrictor pathway and the nerves to the ocular muscles in manBrain69301309
[34] KerrFWLHollowellOW1964Location of pupillomotor and accommodation fibers in the oculomotor nerves: experimental observations of paralytic mydriasisJ Neurol Neurosurg Psychiatry27473481
[35] MustafaGYGambleHJ1979Changes in axonal numbers in developing human trochlear nerveJ Anat128323330
[36] SchuzAPalmG1989Density of neurons and synapses in the cerebral cortex of the mouseJ Comp Neurol286442445
[37] MiettinenRAKalesnykasGKoivistoEH2002Estimation of the total number of cholinergic neurons containing estrogen receptor- in the rat basal forebrainJ Histochem Cytochem50891902
[38] EdwardsKGriffithsDMorganJPitmanRvon RuhlandC2009Can the choice of intermediate solvent or resin affect glomerular basement membrane thickness?Nephrol Dial Transplant24400403
[39] SawabeYMatsumotoKGotoNOtsukaNKobayashiN1998Morphometric nerve fiber analysis and aging process of the human abducent nerveOkajimas Folia Anat Jpn74337343
[40] GeunaS2000Appreciating the difference between design-based and model-based sampling strategies in quantitative morphology of the nervous systemJ Comp Neurol427333339
[41] KaplanSGeunaSRonchiGUlkayMBvon BartheldCS2010Calibration of the stereological estimation of the number of myelinated axons in the rat sciatic nerve: A multicenter studyJ Neurosci Methods1879099
[42] SchroderJMBohlJVon BardelebenU1988Changes of the ratio between myelin thickness and axon diameter in human developing sural, femoral, ulnar, facial and trochlear nervesActa Neuropathol76471483
[43] PetersA2002The effects of normal aging on myelin and nerve fibers: a reviewJ Neurocytol31581593
[44] SturrockRR1989Stability of neuron and glial number in the abducens nerve nucleus of the ageing mouse brainJ Anat16697101
[45] HuamanAGSharpeJA1993Vertical saccades in senescenceInvest Ophthalmol Vis Sci3425882595
[46] MoschnerCBalohRW1994Age-related changes in visual trackingJ Gerontol49M235238
[47] OguroHOkadaKSuyamaNYamashitaKYamaguchSKobayashiS2004Decline of vertical gaze and convergence with agingGerontology50177178
[48] QingYKapoulaZ2004Saccade-vergence dynamics and interaction in children and adultsExp Brain Res156212223
[49] RamboldHNeumanGSanderTHelmchenC2005Age-related changes of vergence under natural viewing conditionsNeurobiol Aging27163172
[50] YangQKapoulaZ2008Aging does not affect the accuracy of vertical saccades nor the quality of their binocular coordination: a study of a special elderly groupNeurobiol Aging29622638
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