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Aging and Disease    2015, Vol. 6 Issue (6) : 456-465     DOI: 10.14336/AD.2015.0327
Original Article |
Genistein, the Isoflavone in Soybean, Causes Amyloid Beta Peptide Accumulation in Human Neuroblastoma Cell Line: Implications in Alzheimer's Disease
Gargi Chatterjee,Debashree Roy,Vineet Kumar Khemka,Mrittika Chattopadhyay,Sasanka Chakrabarti*
Department of Biochemistry, Institute of Post Graduate Medical Education and Research, 244 AJC Bose Road, Kolkata-700020, India.
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Abstract  

The isoflavone, genistein, present in soybean is being actively investigated for its potential beneficial effect against Alzheimer’s disease. Our data, however, show that in SHSY5Y cells genistein causes increased expression (mRNA and protein) of amyloid precursor protein (APP), increased mRNA expression and activity of β-secretase and diminished level of insulin degrading enzyme (IDE) which also degrades amyloid beta peptide. These effects of genistein lead to enhanced accumulation of amyloid beta peptide (Aβ42) in SHSY5Y cells. The results do not support the view that genistein could be a putative drug against AD and instead strengthen the epidemiological study which implies that genistein content of soybean food product (Tofu) leads to cognitive impairment.

Keywords Alzheimer’s disease      genistein      amyloid precursor protein      β-secretase      insulin degrading enzyme      amyloid β42.     
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Issue Date: 01 December 2015
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Gargi Chatterjee
Debashree Roy
Vineet Kumar Khemka
Mrittika Chattopadhyay
Sasanka Chakrabarti
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Gargi Chatterjee,Debashree Roy,Vineet Kumar Khemka, et al. Genistein, the Isoflavone in Soybean, Causes Amyloid Beta Peptide Accumulation in Human Neuroblastoma Cell Line: Implications in Alzheimer's Disease[J]. A&D, 2015, 6(6): 456-465.
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http://www.aginganddisease.org/EN/10.14336/AD.2015.0327     OR     http://www.aginganddisease.org/EN/Y2015/V6/I6/456
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