Please wait a minute...
 Home  About the Journal Editorial Board Aims & Scope Peer Review Policy Subscription Contact us
 
Early Edition  //  Current Issue  //  Open Special Issues  //  Archives  //  Most Read  //  Most Downloaded  //  Most Cited
Aging and disease    2017, Vol. 8 Issue (1) : 1-6     DOI: 10.14336/AD.2016.0625
Short Communication |
Imaging and Quantitative Analysis of the Interstitial Space in the Caudate Nucleus in a Rotenone-Induced Rat Model of Parkinson’s Disease Using Tracer-based MRI
Lv Deyong1,2,3, Li Jingbo4, Li Hongfu3, Fu Yu5,*, Wang Wei1,2,*
1Department of Radiology, Peking University Third Hospital, Beijing 100191, China
2Beijing Key Laboratory of Magnetic Resonance Imaging Device and Technique, Beijing 100191, China
3Department of Radiology, Dongying People’s Hospital of Shandong, Shandong, 257091, China
4Department of Ultrasound, Dongying People’s Hospital of Shandong, Shandong, 257091, China
5Department of Neurology, Peking University Third Hospital, Beijing 100191, China
Download: PDF(1155 KB)   HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks    
Abstract  

Parkinson’s disease (PD) is characterized by pathological changes within several deep structures of the brain, including the substantia nigra and caudate nucleus. However, changes in interstitial fluid (ISF) flow and the microstructure of the interstitial space (ISS) in the caudate nucleus in PD have not been reported. In this study, we used tracer-based magnetic resonance imaging (MRI) to quantitatively investigate the alterations in ISS and visualize ISF flow in the caudate nucleus in a rotenone-induced rat model of PD treated with and without madopar. In the rotenone-induced rat model, the ISF flow was slowed and the tortuosity of the ISS was significantly decreased. Administration of madopar partially prevented these changes of ISS and ISF. Therefore, our data suggest that tracer-based MRI can be used to monitor the parameters related to ISF flow and ISS microstructure. It is a promising technique to investigate the microstructure and functional changes in the deep brain regions of PD.

Keywords Magnetic Resonance      Parkinson’s disease      Interstitial Space      Brain      Diffusion     
Corresponding Authors: Fu Yu,Wang Wei   
Issue Date: 01 February 2017
Service
E-mail this article
E-mail Alert
RSS
Articles by authors
Lv Deyong
Li Jingbo
Li Hongfu
Fu Yu
Wang Wei
Cite this article:   
Lv Deyong,Li Jingbo,Li Hongfu, et al. Imaging and Quantitative Analysis of the Interstitial Space in the Caudate Nucleus in a Rotenone-Induced Rat Model of Parkinson’s Disease Using Tracer-based MRI[J]. Aging and disease, 2017, 8(1): 1-6.
URL:  
http://www.aginganddisease.org/EN/10.14336/AD.2016.0625     OR     http://www.aginganddisease.org/EN/Y2017/V8/I1/1
Figure 1.  Behavioral changes after treatment. (A) The average hanging time in the hanging-wire test four weeks after rotenone-induced damage and madopar treatment. (B) The average inclination angle in the inclined plane test four weeks after rotenone-induced damage and madopar treatment. Both the hanging time and the inclination angle were significantly decreased in the rotenone-induced group compared to the other two groups. Data are the mean ± SEM (n = 10). One-way ANOVA and SNK tests were performed, * represents P < 0.05.
Figure 2.  Axial views of MRI and spatiotemporal distribution pattern of Gd-DTPA after injection into caudate nucleus. (A) Axial MR images of the spreading tracer in the ISS at different time points after the introduction of Gd-DTPA. Gd-DTPA introduced into the ISS can lighten the water molecules and increase the signal intensity in the spreading regions. The transportation and clearance of Gd-DTPA can be demonstrated using a series of MR images. (B) Line chart of the distribution region at the different time points. The volume of each pixel in the MRI is 0.5×0.5×0.5 mm3, and the volume amount of the “lightened” regions in each image for each time point was calculated. The maximum spreading region was not significantly different among the three groups. Data are the mean ± SEM (n=10). SNK test was performed.
Figure 3.  Alterations in the microstructure parameters (D*, λ) and the clearance of Gd-DTPA (k’, t½) in the caudate nucleus after rotenone-induced damage and madopar treatment. (A) Compared to the sham group, the effective diffusion parameter(D*) was significantly increased in the rotenone-induced group. No differences were observed between the sham group and the madopar-treated group. (B) Compared to the sham group, tortuosity (λ) was significantly decreased in the rotenone-induced group. No differences were observed between the sham group and the madopar-treated group. (C) Compared to the sham group, the clearance rate constant (k’) was significantly decreased in the rotenone-induced group. No differences were observed between the sham group and the madopar-treated group. (D) Compared to the sham group, half-life (t½) was significantly prolonged in the rotenone-induced group. No differences were observed between the sham group and the madopar-treated group. Data are the mean ± SEM (n=10); SNK test was performed. * represents P<0.05
[1] Lei Y, Han H, Yuan F, Javeed A, Zhao Y (2016). The brain interstitial system: Anatomy, modeling, in vivo measurement, and applications. Prog Neurobiol, in press.
[2] Labandeira-Garcia JL, Rodriguez-Pallares J, Villar-Cheda B, Rodriguez-Perez AI, Garrido-Gil P, Guerra MJ (2011). Aging, Angiotensin system and dopaminergic degeneration in the substantia nigra. Aging Dis, 2:257-274
[3] Rangel-Barajas C, Coronel I, Floran B (2015). Dopamine Receptors and Neurodegeneration. Aging Dis, 6:349-368
[4] Duarte JMN, Schuck PF, Wenk GL, Ferreira GC (2014). Metabolic Disturbances in Diseases with Neurological Involvement. Aging Dis, 5:238-255
[5] Ren RT, Shi CY, Cao J, Sun Y, Zhao X, Guo YF, et al (2016). Neuroprotective Effects of A Standardized Flavonoid Extract of Safflower Against Neurotoxin-Induced Cellular and Animal Models of Parkinson’s Disease. Sci Rep, 6:22135
[6] Reum T, Olshausen F, Mazel T, Vorisek I, Morgenstern R, Sykova E (2002). Diffusion parameters in the striatum of rats with 6-hydroxydopamine-induced lesions and with fetal mesencephalic grafts. J Neurosci Res, 70: 680-693
[7] San Sebastian W, Richardson RM, Kells AP, Lamarre C, Bringas J, Pivirotto P, et al (2012). Safety and tolerability of magnetic resonance imaging-guided convection-enhanced delivery of AAV2-hAADC with a novel delivery platform in nonhuman primate striatum. Hum Gene Ther, 23:210-217
[8] Kells AP, Eberling J, Su X, Pivirotto P, Bringas J, Hadaczek P, et al (2010). Regeneration of the MPTP-lesioned dopaminergic system after convection-enhanced delivery of AAV2-GDNF. J Neurosci, 30: 9567-9577
[9] Sykova E, Nicholson C (2008). Diffusion in brain extracellular space. Physiol Rev, 88:1277-1340
[10] Han H, Shi C, Fu Y, Zuo L, Lee K, He Q, et al (2014). A novel MRI tracer-based method for measuring water diffusion in the extracellular space of the rat brain. IEEE J Biomed Health Inform, 18:978-983
[11] Li K, Han H, Zhu K, Lee K, Liu B, Zhou F, et al (2013). Real-time magnetic resonance imaging visualization and quantitative assessment of diffusion in the cerebral extracellular space of C6 glioma-bearing rats. Neurosci Lett, 543:84-89
[12] Fathalla AM, Soliman AM, Ali MH, Moustafa AA (2016). Adenosine A2A Receptor Blockade Prevents Rotenone-Induced Motor Impairment in a Rat Model of Parkinsonism. FrontBehav Neurosci, 10:35
[13] von Wrangel C, Schwabe K, John N, Krauss JK, Alam M (2015). The rotenone-induced rat model of Parkinson’s disease: behavioral and electrophysiological findings. Behav Brain Res, 279: 52-61
[14] Field EF, Whishaw IQ, Pellis SM (2000). Sex differences in catalepsy: evidence for hormone-dependent postural mechanisms in haloperidol-treated rats. Behavioural brain research, 109:207-212
[15] Han HB, Li K, Yan JH, Zhu K, Fu Y (2012). An in vivo study with an MRI tracer method reveals the biophysical properties of interstitial fluid in the rat brain. Sci China Life Sci, 55:782-787
[16] Paxinos G, Watson C, editors. The Rat Brain in Stereotaxic Coordinates. London: Academic Press; 2007
[17] Liu B, Bai XZ, Zhou FG, Han HB, Hou C (2013). Mutual information based three-dimensional registration of rat brain magnetic resonance imaging time-series. Comput Electr Eng, 39:1473-1484
[18] Iliff JJ, Wang M, Liao Y, Plogg BA, Peng W, Gundersen GA, et al (2012). A paravascular pathway facilitates CSF flow through the brain parenchyma and the clearance of interstitial solutes, including amyloid beta. Sci Transl Med, 4: 147ra111
[19] Xiao FR, Nicholson C, Hrabe J, Hrabetova S (2008). Diffusion of flexible random-coil dextran polymers measured in anisotropic brain extracellular space by integrative optical Imaging. Biophys J, 95:1382-1392
[20] Gates MA, Laywell ED, Fillmore H, Steindler DA (1996). Astrocytes and extracellular matrix following intracerebral transplantation of embryonic ventral mesencephalon or lateral ganglionic eminence. Neuroscience, 74:579-597
[21] Burke RE, O’Malley K (2013). Axon degeneration in Parkinson’s disease. Exp Neuro, 246: 72-83
[22] Shi C, Lei Y, Han H, Zuo L, Yan J, He Q, et al (2015). Transportation in the Interstitial Space of the Brain Can Be Regulated by Neuronal Excitation. Sci Rep, 5: 17673
[23] Davie CA (2008). A review of Parkinson’s disease. Br Med Bull, 86:109-127
[24] Chen ZG (2015). Cell Therapy for Parkinson’s Disease: New Hope from Reprogramming Technologies. Aging Dis, 6:499-503
[25] Stoessl AJ (2014). Gene therapy for Parkinson’s disease: a step closer? Lancet, 383: 1107-1109
[26] Xu F, Hongbin H, Yan J, Chen H, He Q, Xu W, et al (2011). Greatly improved neuroprotective efficiency of citicoline by stereotactic delivery in treatment of ischemic injury. Drug Deliv, 18:461-467
[1] Wanying Duan, Yuehua Pu, Haiyan Liu, Jing Jing, Yuesong Pan, Xinying Zou, Yilong Wang, Xingquan Zhao, Chunxue Wang, Yongjun Wang, Ka Sing Lawrence Wong, Ling Wei, Liping Liu, . Association between Leukoaraiosis and Symptomatic Intracranial Large Artery Stenoses and Occlusions: the Chinese Intracranial Atherosclerosis (CICAS) Study[J]. Aging and disease, 2018, 9(6): 1074-1083.
[2] Calvin Pak-Wing Cheng, Sheung-Tak Cheng, Cindy Woon-Chi Tam, Wai-Chi Chan, Winnie Chiu-Wing Chu, Linda Chiu-Wa Lam. Relationship between Cortical Thickness and Neuropsychological Performance in Normal Older Adults and Those with Mild Cognitive Impairment[J]. Aging and disease, 2018, 9(6): 1020-1030.
[3] Ze Teng, Aibo Wang, Peng Wang, Rui Wang, Wei Wang, Hongbin Han. The Effect of Aquaporin-4 Knockout on Interstitial Fluid Flow and the Structure of the Extracellular Space in the Deep Brain[J]. Aging and disease, 2018, 9(5): 808-816.
[4] Yong-Fei Zhao, Qiong Zhang, Jian-Feng Zhang, Zhi-Yin Lou, Hen-Bing Zu, Zi-Gao Wang, Wei-Cheng Zeng, Kai Yao, Bao-Guo Xiao. The Synergy of Aging and LPS Exposure in a Mouse Model of Parkinson’s Disease[J]. Aging and disease, 2018, 9(5): 785-797.
[5] De Lazzari Federica, Bubacco Luigi, Whitworth Alexander J, Bisaglia Marco. Superoxide Radical Dismutation as New Therapeutic Strategy in Parkinson’s Disease[J]. Aging and disease, 2018, 9(4): 716-728.
[6] Zhang Li, Hao Junwei, Zheng Yan, Su Ruijun, Liao Yajin, Gong Xiaoli, Liu Limin, Wang Xiaomin. Fucoidan Protects Dopaminergic Neurons by Enhancing the Mitochondrial Function in a Rotenone-induced Rat Model of Parkinson’s Disease[J]. Aging and disease, 2018, 9(4): 590-604.
[7] Chen Yali, Yin Mengmei, Cao Xuejin, Hu Gang, Xiao Ming. Pro- and Anti-inflammatory Effects of High Cholesterol Diet on Aged Brain[J]. Aging and disease, 2018, 9(3): 374-390.
[8] Sun Wenzhi, Tan Jiewen, Li Zhuo, Lu Shibao, Li Man, Kong Chao, Hai Yong, Gao Chunjin, Liu Xuehua. Evaluation of Hyperbaric Oxygen Treatment in Acute Traumatic Spinal Cord Injury in Rats Using Diffusion Tensor Imaging[J]. Aging and disease, 2018, 9(3): 391-400.
[9] Zhang Can, Brandon Nicole R., Koper Kerryann, Tang Pei, Xu Yan, Dou Huanyu. Invasion of Peripheral Immune Cells into Brain Parenchyma after Cardiac Arrest and Resuscitation[J]. Aging and disease, 2018, 9(3): 412-425.
[10] Perez-Roca Laia, Adame-Castillo Cristina, Campdelacreu Jaume, Ispierto Lourdes, Vilas Dolores, Rene Ramon, Alvarez Ramiro, Gascon-Bayarri Jordi, Serrano-Munoz Maria A., Ariza Aurelio, Beyer Katrin. Glucocerebrosidase mRNA is Diminished in Brain of Lewy Body Diseases and Changes with Disease Progression in Blood[J]. Aging and disease, 2018, 9(2): 208-219.
[11] Zhang Meng, Deng Yong-Ning, Zhang Jing-Yi, Liu Jie, Li Yan-Bo, Su Hua, Qu Qiu-Min. SIRT3 Protects Rotenone-induced Injury in SH-SY5Y Cells by Promoting Autophagy through the LKB1-AMPK-mTOR Pathway[J]. Aging and disease, 2018, 9(2): 273-286.
[12] Zhang Lin-Yuan, Lin Pan, Pan Jiaji, Ma Yuanyuan, Wei Zhenyu, Jiang Lu, Wang Liping, Song Yaying, Wang Yongting, Zhang Zhijun, Jin Kunlin, Wang Qian, Yang Guo-Yuan. CLARITY for High-resolution Imaging and Quantification of Vasculature in the Whole Mouse Brain[J]. Aging and disease, 2018, 9(2): 262-272.
[13] Peng Fangyu, Xie Fang, Muzik Otto. Alteration of Copper Fluxes in Brain Aging: A Longitudinal Study in Rodent Using 64CuCl2-PET/CT[J]. Aging and disease, 2018, 9(1): 109-118.
[14] Sun Qian, Wang Tian, Jiang Tian-Fang, Huang Pei, Wang Ying, Xiao Qin, Liu Jun, Chen Sheng-Di. Clinical Profile of Chinese Long-Term Parkinson’s Disease Survivors With 10 Years of Disease Duration and Beyond[J]. Aging and disease, 2018, 9(1): 8-16.
[15] Farnaz Farokhian,Chunlan Yang,Iman Beheshti,Hiroshi Matsuda,Shuicai Wu. Age-Related Gray and White Matter Changes in Normal Adult Brains[J]. A&D, 2017, 8(6): 899-909.
Viewed
Full text


Abstract

Cited

  Shared   
Copyright © 2014 Aging and Disease, All Rights Reserved.
Address: Aging and Disease Editorial Office 3400 Camp Bowie Boulevard Fort Worth, TX76106 USA
Fax: (817) 735-0408 E-mail: editorial@aginganddisease.org
Powered by Beijing Magtech Co. Ltd