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Aging and disease    2018, Vol. 9 Issue (2) : 317-321     DOI: 10.14336/AD.2017.0630
Short Communications |
Serum Zonulin and Endotoxin Levels in Exceptional Longevity versus Precocious Myocardial Infarction
Carrera-Bastos Pedro1,2, Picazo Óscar2, Fontes-Villalba Maelán1,2, Pareja-Galeano Helios3,4,*, Lindeberg Staffan1, Martínez-Selles Manuel5, Lucia Alejandro3,4, Emanuele Enzo6
1Clinical Research Center, Faculty of Medicine, Center for Primary Health Care Research, Lund University, Malmö, Sweden.
2NutriScience - Education and Consulting, Lisbon, Portugal.
3European University of Madrid, Spain.
4Research Institute of Hospital 12 de Octubre (’i+12’), Madrid, Spain.
5Servicio de Cardiología, Hospital Universitario Gregorio Marañón, CIBERCV, Universidad Europea, Universidad Complutense, Madrid, Spain
62E Science, Robbio, Pavia, Italy.
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Endotoxemia-induced inflammation has been associated with insulin resistance and atherosclerosis, ultimately increasing the risk of coronary heart disease. Increased intestinal permeability is an important event leading to endotoxemia. This study aims to elucidate the possible association between endotoxin (lipopolysaccharide) and zonulin (a biomarker of intestinal permeability) levels and the risk of coronary heart disease, and thus healthy aging. Serum levels of zonulin, lipopolysaccharide and soluble CD14 (a protein that binds lipopolysaccharide) were measured in disease-free centenarians, young healthy controls and patients with precocious acute myocardial infarction. Disease-free centenarians had significantly lower levels of serum zonulin (P<0.01) and lipopolysaccharide (P<0.001) than young patients with acute myocardial infarction, and had significantly lower concentrations of serum lipopolysaccharide than young healthy controls (P<0.05). No significant differences were found for soluble CD14 between groups. Our findings may stimulate further research into the role played by intestinal permeability and endotoxemia not only in coronary heart disease but also in lifespan modulation.

Keywords centenarians      longevity      inflammation      endotoxemia     
Corresponding Authors: Pareja-Galeano Helios   
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These authors contributed equally to this work.

Issue Date: 01 April 2018
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Carrera-Bastos Pedro
Picazo Óscar
Fontes-Villalba Maelán
Pareja-Galeano Helios
Lindeberg Staffan
Martínez-Selles Manuel
Lucia Alejandro
Emanuele Enzo
Cite this article:   
Carrera-Bastos Pedro,Picazo Óscar,Fontes-Villalba Maelán, et al. Serum Zonulin and Endotoxin Levels in Exceptional Longevity versus Precocious Myocardial Infarction[J]. Aging and disease, 2018, 9(2): 317-321.
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BiomarkersYoung AMI patientsHealthy young controlsCentenariansP-value
LPS (EU/mL)15.4 ± 5.26.1 ± 7.34.2 ± 5.3<0.001
Soluble CD14 (ng/mL)1,489 ± 3391,438 ± 3121,341 ± 274ns
Zonulin (pg/mL)7.6 ± 3.15.2 ± 2.74.0 ± 2.1<0.001
Table 1  Serum biochemical parameters in the three study groups.
EndotoxinSoluble CD14Zonulin
LPSAMI-ρ = -0.06, nsρ = 0.48, P <0.001
Controls-ρ = 0.09, nsρ = 0.19, ns
Centenarians-ρ = -0.12, nsρ = 0.64, P < 0.001
Soluble CD14AMIρ = -0.06, ns-ρ = 0.12, ns
Controlsρ = 0.09, ns-ρ = 0.08, ns
Centenariansρ = -0.12, ns-ρ = -0.01, ns
ZonulinAMIρ = 0.48, P <0.001r = 0.12, ns-
Controlsρ = 0.19, nsr = 0.08, ns-
Centenariansρ = 0.64, P < 0.001r = -0.01, ns-
Table 2  Correlation coefficients (Spearman’s ρ) between serum biomarkers in the three study groups.
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