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Aging and Disease    2018, Vol. 9 Issue (4) : 623-633     DOI: 10.14336/AD.2017.0901
Orginal Article |
Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes
Feng Tang,Meng-Hao Pan,Yujie Lu,Xiang Wan,Yu Zhang,Shao-Chen Sun
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
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Abstract  

Kif4a, a member of the kinesin superfamily, has been reported to participate in a series of cellular processes such as chromosome condensation and cytokinesis during mitosis. However, the roles of KIF4a in meiosis are still unknown. In present study we found that the Kif4a protein expression decreased in maternal aged mouse oocytes. We then explored the roles of Kif4a in mouse oocyte meiosis by knockdown analysis. Kif4a was enriched at the spindle during mouse oocyte maturation. By specific knock down of the Kif4a using morpholino microinjection, we found that the disruption of Kif4a caused the failure of polar body extrusion. Further analysis indicated that Kif4a might affect the spindle morphology and chromosome alignment in the mouse oocytes, and this might be due to the regulation of tubulin acetylation. Moreover, our results showed that an increased proportion of aneuploidy in the Kif4a knock down oocytes, and this might be due to the loss of kinetochore-microtubule attachment. Taken together, these results suggested that Kif4a possibly regulated mouse oocyte meiosis through its effects on the spindle organization and accurate chromosome segregation, and the loss of Kif4a might be related with aneuploidy of aging oocytes.

Keywords Kif4a      oocyte      meiosis      aneuploidy      aging     
Corresponding Authors: Shao-Chen Sun   
Issue Date: 01 August 2018
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Feng Tang
Meng-Hao Pan
Yujie Lu
Xiang Wan
Yu Zhang
Shao-Chen Sun
Cite this article:   
Feng Tang,Meng-Hao Pan,Yujie Lu, et al. Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes[J]. A&D, 2018, 9(4): 623-633.
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http://www.aginganddisease.org/EN/10.14336/AD.2017.0901     OR     http://www.aginganddisease.org/EN/Y2018/V9/I4/623
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