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Aging and Disease    2018, Vol. 9 Issue (4) : 566-577     DOI: 10.14336/AD.2017.1001
Orginal Article |
Effect of Aging and Glucagon-like Peptide 2 on Intestinal Microbiota in SD Rats
Jiayu Wu1,Weiying Ren1,Li Li2,Man Luo1,Kan Xu1,Jiping Shen1,Jia Wang1,Guilin Chang1,Yi Lu1,Yiming Qi2,Binger Xu1,Yuting He1,Yu Hu1,*
1Department of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai 200032, China
2Department of Internal Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China
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Abstract  

Recent research suggests that intestinal microbiota affect the aging process. Glucagon-like peptide 2 (GLP-2), a growth factor found in the intestinal mucosal epithelium, reduces intestinal permeability and affects intestinal microbiota. The relationship between aging, GLP-2, and intestinal microbiota are still not well understood. The current study examined the influence of aging and GLP-2 on the intestinal microbiota of rats. Twelve 3-month old male SD rats were randomly divided into two groups: a young control group (group C) and a young GLP-2 treatment group (group G). Twelve 26-month old male SD rats were randomly divided into two groups: an aged control group (group L) and an aged GLP-2 treatment group (group T). GLP-2 was intraperitoneally injected into rats from group G and group T for 14 days. Plasma GLP-2 concentration was evaluated by ELISA tests. Fresh intestinal stool samples were collected from each group and total fecal bacterial genomic DNA was extracted from the associated rats. The bacterial composition of fecal samples was analyzed by Miseq high-throughput sequencing and comparison with SRA databases. Overall, the diversity of intestinal microbiota significantly decreases with age in SD rats, while GLP-2 has no significant effect on the diversity of intestinal microbiota. Upon aging, there is a reduction in probiotic bacteria and a concomitant increase in pathogenic bacteria in rats. Treatment with GLP-2 results in a significant reduction in the prevalence of pathogenic bacterial genera and an increase in some potential benefit bacteria in aged rats. In addition, treatment with GLP-2 results in an increase in several probiotics and a reduction in the prevalence of pathogenic bacterial genera in young rats.

Keywords aging      GLP-2      intestinal microbiota      high-throughput sequencing     
Corresponding Authors: Yu Hu   
Issue Date: 01 August 2018
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Jiayu Wu
Weiying Ren
Li Li
Man Luo
Kan Xu
Jiping Shen
Jia Wang
Guilin Chang
Yi Lu
Yiming Qi
Binger Xu
Yuting He
Yu Hu
Cite this article:   
Jiayu Wu,Weiying Ren,Li Li, et al. Effect of Aging and Glucagon-like Peptide 2 on Intestinal Microbiota in SD Rats[J]. A&D, 2018, 9(4): 566-577.
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http://www.aginganddisease.org/EN/10.14336/AD.2017.1001     OR     http://www.aginganddisease.org/EN/Y2018/V9/I4/566
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