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Aging and Disease    2018, Vol. 9 Issue (1) : 109-118     DOI: 10.14336/AD.2017.1025
Orginal Article |
Alteration of Copper Fluxes in Brain Aging: A Longitudinal Study in Rodent Using 64CuCl2-PET/CT
Fangyu Peng1,2,*,Fang Xie1,Otto Muzik3,4
1Department of Radiology, and
2Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX75390, USA
3Department of Pediatrics and
4 Department of Radiology, Wayne State University, Detroit, MI 48202, USA
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Abstract  

Brain aging is associated with changes of various metabolic pathways. Copper is required for brain development and function, but little is known about changes in copper metabolism during brain aging. The objective of this study was to investigate alteration of copper fluxes in the aging mouse brain with positron emission tomography/computed tomography using 64CuCl2 as a radiotracer (64CuCl2-PET/CT). A longitudinal study was conducted in C57BL/6 mice (n = 5) to measure age-dependent brain and whole-body changes of 64Cu radioactivity using PET/CT after oral administration of 64CuCl2 as a radiotracer. Cerebral 64Cu uptake at 13 months of age (0.17 ± 0.05 %ID/g) was higher than the cerebral 64Cu uptake at 5 months of age (0.11 ± 0.06 %ID/g, p < 0.001), followed by decrease to (0.14 ± 0.04 %ID/g, p = 0.02) at 26 months of age. In contrast, cerebral 18F-FDG uptake was highest at 5 months of age (7.8 ± 1.2 %ID/g) and decreased to similar values at 12 (5.2 ± 1.1 %ID/g, p < 0.001) and 22 (5.6 ± 1.1 %ID/g, p < 0.001) months of age. The findings demonstrated alteration of copper fluxes associated with brain aging and the time course of brain changes in copper fluxes differed from changes in brain glucose metabolism across time, suggesting independent underlying physiological processes. Hence, age-dependent changes of cerebral copper fluxes might represent a novel metabolic biomarker for assessment of human brain aging process with PET/CT using 64CuCl2 as a radiotracer.

Keywords Positron emission tomography      brain aging      Alzheimer’s disease      copper fluxes      glucose metabolism      copper-64 chloride     
Corresponding Authors: Fangyu Peng   
Issue Date: 01 February 2018
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Fangyu Peng
Fang Xie
Otto Muzik
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Fangyu Peng,Fang Xie,Otto Muzik. Alteration of Copper Fluxes in Brain Aging: A Longitudinal Study in Rodent Using 64CuCl2-PET/CT[J]. A&D, 2018, 9(1): 109-118.
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http://www.aginganddisease.org/EN/10.14336/AD.2017.1025     OR     http://www.aginganddisease.org/EN/Y2018/V9/I1/109
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