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Aging and disease    2018, Vol. 9 Issue (5) : 798-807     DOI: 10.14336/AD.2017.1223
Orginal Article |
Age-related Deficits in Recognition Memory are Protocol-Dependent
Diano F. Marrone1,2,*,  Elham Satvat3, Anuj Patel1
1Dept. of Psychology, Wilfrid Laurier University, Waterloo, ON N2L 3C5, Canada
2McKnight Brain Institute, University of Arizona, Tucson, AZ 85724, USA,
3School of Public Health & Health Systems, University of Waterloo, Waterloo, ON N2L 3G1, Canada
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Abstract  

The perirhinal cortex (PRh) is a critical mediator of recognition memory, and a wealth of evidence points to impairment in PRh function with age. Despite this evidence, age-related deficits in recognition memory are not consistently observed. This may be partially due to the fact that older animals also have well-established deficits in hippocampal function, and many protocols that assess perirhinal function are also sensitive to hippocampal damage. When using one of these protocols, spontaneous object recognition in an open field, we are able to replicate published age-related deficits using pairs of complex objects. However, when using zero-delay object recognition, a task that is more resistant to the influence of changes in hippocampal function, we find no significant age-related differences in recognition memory in the same animals. These data highlight the importance of the protocol used for testing recognition memory, and may place constraints on the role of the PRh in age-related recognition memory impairment as it is typically tested in much of the literature.

Keywords recognition memory      aging      hippocampus      perirhinal cortex     
Corresponding Authors: Marrone Diano F.   
About author: These authors contributed equally to this work.
Issue Date: 26 September 2017
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Marrone Diano F.
Satvat Elham
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Marrone Diano F.,Satvat Elham,Patel Anuj. Age-related Deficits in Recognition Memory are Protocol-Dependent[J]. Aging and disease, 2018, 9(5): 798-807.
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http://www.aginganddisease.org/EN/10.14336/AD.2017.1223     OR     http://www.aginganddisease.org/EN/Y2018/V9/I5/798
Age (months)TaskConditionNovel object exploration time (sec)1Familiar object exploration time (sec)1
4-6SOREasy25.29 ± 4.8619.29 ± 2.15
Hard19.57 ± 2.9421.19 ± 3.66
ZOREasy18.75 ± 4.1719.12 ± 6.81
Hard18.01 ± 1.8917.11 ± 2.42
11-13SOREasy29.4 ± 5.5823.2 ± 2.99
Hard19.0 ± 2.0918.4 ± 3.01
ZOREasy22.29 ± 3.7618.29 ± 5.05
Hard29.1 ± 7.7524.88 ± 11.40
23-25SOREasy22.56 ± 3.5024.11 ± 6.87
Hard25.78 ± 7.8223.1 ± 10.89
ZOREasy19.45 ± 4.1718.72 ± 6.81
Hard21.09 ± 7.6721.00 ± 10.54
Table 1  Mean object exploration time across all conditions
Figure 1.  Changes in testing protocol determine the presence of age-related recognition memory deficits

Examples of object pairs used are shown (A). All objects were junk items purchased from local stores and included dog toys, children’s toys, and small household decorative items such as candlesticks. The procedures (B) for zero-delay object recognition (ZOR, left) and spontaneous object recognition (SOR, right) are depicted. In ZOR, sample trial 1 consists of 2 identical pairs of objects pairs (AB) presented in each arm of a y-maze that remained until the animal had explored one of the objects for at least 25 sec. After this criterion is met, barriers are removed to reveal a new identical pair of objects (CD). Once the rat explored one of these objects for at least 25 sec, the second barrier was removed to reveal a familiar (AB) object and a novel pair of two unique objects (EF, easy condition), or a novel combination of previously seen objects (AC, hard). The SOR task uses similar methodology in an open field. In SOR, sample trial 1 consists of 2 identical pairs of objects (GH) presented within an open field until the animal had explored one of the objects for at least 25 sec. The rat was then removed from the open field for 120 sec, while a new identical pair of object was placed in the field (IJ). The rat returned to the field for sample trial 2 until it explored one of the objects for at least 25 sec. After a 120 sec delay, rats were then tested with a familiar (GH) object and a novel pair of two unique objects (KL, easy condition), or a novel combination of previously seen objects (HI, hard). Quantitative analyses of the ZOR (C) and SOR (D) reveal different effects obtained from these protocols. While 6-month-old (white bars) and 12-month-old (light grey) animals generally perform well under all conditions, 24-month old animals (dark grey) show recognition memory deficits only in SOR, while their performance in ZOR is relatively intact (data are mean ± SEM, * = p < 0.05 vs 12 months, ‡ = p < 0.05 vs. 24 months, § = p <0.05 vs. easy trials in the same age group).

Figure 2.  Age-related spatial memory deficits correlate with spontaneous object recognition performance

Analysis of path lengths (A) in the Morris water maze (MWM) shows that when the platform was hidden, 6-month-old rats (white diamonds) swam shorter paths to reach the hidden platform than either 12-month-old (light gray square) 24-month-old (dark gray triangle) rats by day 2 of training. By day 4, 12-month-old rats also outperformed 24-month old ones. During trials in which the platform was visible (B), all 3 age groups had significantly different path lengths on day one, and this difference became much smaller by day 2 such that only 6-month-old and 24-month-old rats shows a significant difference. During the probe trial (C), both 6-month-old (white) and 12-month-old (light grey) rats spent significantly more time than aged rats (dark grey) in the quadrant that previously held the platform (target) than the opposite quadrant. Regression shows that spatial memory performance does not predict the performance of individual animals in zero-delay object recognition (ZOR, D). However, spatial memory significantly predicts SOR performance (E) in individual animals (all data are mean ± SEM; * = p < 0.05, 12 vs 24-moth-old; † = p < 0.05, 6-month-old vs 24-month-old; ‡ = p < 0.05, 6-month-old and 24-month-old; § p < 0.05, vs opposite quadrant in the same age group).

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