Aging and disease

ISOAD Become a Member Search

X

Advanced Search

Table of Content

01 April 2013. VOL 4, ISSUE 4 Previous Issue Next Issue

Differential Effect of Aging on Verbal and Visuo-Spatial Working Memory
Navnit Kumar, Brajesh Priyadarshi

2013, 4 (4): 170-178

PDF (735KB)
Validated Liquid Culture Monitoring System for Lifespan Extension of Caenorhabditis elegans through Genetic and Dietary Manipulations
Myat Thu Thu Win, Yasuhiko Yamamoto, Seiichi Munesue, Dong Han, Shin-ichi Harada, Hiroshi Yamamoto

2013, 4 (4): 178-185

PDF (635KB)
Functional Analysis and Molecular Docking studies of Medicinal Compounds for AChE and BChE in Alzheimer’s Disease and Type 2 Diabetes Mellitus
Dowluru SVGK Kaladhar, Nagendra Sastry Yarla, N. Anusha

2013, 4 (4): 186-200

PDF (914KB)
Older Subjects without Radiographic Knee Osteoarthritis: Weight, Height, and Body Mass Index
Lissa Fahlman, Emmeline Sangeorzan, Nimisha Chheda

2013, 4 (4): 201-209

PDF (523KB)
Alzheimer’s Disease and Immunotherapy
Jennifer Madeo, Marianne Frieri

2013, 4 (4): 210-220

PDF (535KB)
Aging, Neurogenesis, and Caloric Restriction in Different Model Organisms
Ayca Arslan-Ergul, A Tugrul Ozdemir, Michelle M Adams

2013, 4 (4): 221-232

PDF (546KB)

Cover Illustration

01 April 2013. Vol.4 No.4
Nutritional and genetic factors influence aging and life expectancy. The reduction of food intake without malnutrition, referred to caloric restriction (CR), has been shown to increase lifespan in a wide variety of species. The nematode Caenorhabditis elegans (C. elegans) is one of the principle models with which to study the biology of aging and search for anti-aging compounds. In this study, we validated and optimized a high-throughput liquid culture system to monitor C. elegans lifespan with minimized mechanical stress. We used alive and ultraviolet (UV)-killed Escherichia coli (E. coli) OP50 at 10⁸ or 10⁹ colony-forming units (cfu)/ml to feed Bristol N2 wild-type (WT) and mutant worms of a well-characterized insulin/insulin-like growth factor signaling (ILS) pathway: the insulin receptor homolog daf-2 (e1370), phosphatidylinositol 3-kinase age-1 (hx546), and transcriptional factor FOXO homolog daf-16 (mu86 and mgDf50). Compared with alive E. coli at 10⁹ cfu/ml, supplementations of alive E. coli at 10⁸ cfu/ml or UV-killed E. coli at 10⁹ cfu/ml dramatically prolonged lifespan in WT and age-1 mutants, and to a lesser extent, in daf-2 and daf-16 mutants, suggesting that signaling pathways in CR and ILS do not overlap fully. Feeding 10⁸ cfu/ml UV-killed E. coli, which led to maximally saturated longevity in WT and daf-2 mutant, can prolonged lifespan in age-1, but not daf-16, mutants. This approach will be useful for investigating the biology of aging, physiological responses and gene functions under CR conditions and also for screening pharmacologic compounds to extend lifespan or affect other biologic processes.

[Detail]

Cover Illustration

Nutritional and genetic factors influence aging and life expectancy. The reduction of food intake without malnutrition, referred to caloric restriction (CR), has been shown to increase lifespan in a wide variety of species. The nematode Caenorhabditis elegans (C. elegans) is one of the principle models with which to study the biology of aging and search for anti-aging compounds. In this study, we validated and optimized a high-throughput liquid culture system to monitor C. elegans lifespan with minimized mechanical stress. We used alive and ultraviolet (UV)-killed Escherichia coli (E. coli) OP50 at 10⁸ or 10⁹ colony-forming units (cfu)/ml to feed Bristol N2 wild-type (WT) and mutant worms of a well-characterized insulin/insulin-like growth factor signaling (ILS) pathway: the insulin receptor homolog daf-2 (e1370), phosphatidylinositol 3-kinase age-1 (hx546), and transcriptional factor FOXO homolog daf-16 (mu86 and mgDf50). Compared with alive E. coli at 10⁹ cfu/ml, supplementations of alive E. coli at 10⁸ cfu/ml or UV-killed E. coli at 10⁹ cfu/ml dramatically prolonged lifespan in WT and age-1 mutants, and to a lesser extent, in daf-2 and daf-16 mutants, suggesting that signaling pathways in CR and ILS do not overlap fully. Feeding 10⁸ cfu/ml UV-killed E. coli, which led to maximally saturated longevity in WT and daf-2 mutant, can prolonged lifespan in age-1, but not daf-16, mutants. This approach will be useful for investigating the biology of aging, physiological responses and gene functions under CR conditions and also for screening pharmacologic compounds to extend lifespan or affect other biologic processes.